Journal: The Tohoku Journal of Experimental Medicine

Article Title: Local Delivery of Nimustine Hydrochloride against Brain Tumors: Basic Characterization Study

doi: 10.1620/tjem.2023.j069

Figure Lengend Snippet: Fig. 3. ACNU reduced pFas expression and secretion of TGF-β1 in cells from glioma cell lines in vitro. (A) Each cell line was treated with saline or 10 mM ACNU. Cells were harvested 24 h after treatment and pFas was detected by western blot. Compared with the saline-treated control group, pFas immunoreactivity in ACNU-treated cells was significantly decreased. β-Actin was used as an internal control. (B) Immunoreactivity for pFas was quantitatively analyzed. Bars indicate mean ± standard deviation (SD). *p < 0.05, **p < 0.01. (C) Cells from each tumor cell line were treated with saline (control group) or 10 mM ACNU (treatment group). After 24 h, the supernatant was collected and ELISA was performed to measure TGF-β1. Compared with controls, the amount of secreted TGF-β1 by ACNU- treated cells from each tumor cell line was significantly decreased. Bars indicate mean ± SD. *p < 0.05, **p < 0.01.

Article Snippet: The primary antibodies were a rabbit anti-Fas IgG polyclonal antibody (1:200 dilution; Sc-1023; Santa Cruz Biotechnology, Dallas, TX, USA) and a rabbit anti-βactin IgG monoclonal antibody (1:20,000 dilution; 4970; Cell Signaling Technology, Danvers, MA, USA).

Techniques: Expressing, In Vitro, Saline, Western Blot, Control, Standard Deviation, Enzyme-linked Immunosorbent Assay

Journal: Cancers

Article Title: Partial Hepatectomy Promotes the Development of KRASG12V-Induced Hepatocellular Carcinoma in Zebrafish

doi: 10.3390/cancers16101793

Figure Lengend Snippet: The effect of s100a1 knockdown on the development of kras G12V -induced HCC in zebrafish larvae. ( A ) Experiment design of s100a1 knockdown and oncogene induction in Lipan larvae and kras+ larvae. ( B ) Western blot of S100A1with the total protein extracted from MO-injected zebrafish embryos at 24 hpf. β-actin served as a loading control. Each protein sample was pooled from 15 zebrafish embryos. The quantification of the intensity of S100A1normalized by the intensity of β-actin was at the lower panel. The uncropped image of blots is shown in . ( C ) Fluorescence images of morpholino-treated Lipan and kras+ larvae after 48 h of Dox treatment. ( D ) Measurement of liver size based on the fluorescence in ( B ) ( n ≥ 8). Circles, squares, and triangles indicate values of individual samples ( E ) Expression of sox5, sox9a, and sox9b in the kras+ livers at 24 h after PH/sham surgery as determined by RT-qPCR ( n = 3). Scale Bar = 200 μm. ns p > 0.05, * p ≤ 0.05, ** p ≤ 0.01.

Article Snippet: The effect of MO- s100a1 -ATG on S100A1 expression was validated in 1-dpf zebrafish embryos with western blotting using the Rabbit-anti-βactin antibody (bs-0061R, Bioss Antibodies, Woburn, MA, USA) (1:5000) and the Rabbit-anti-S100A1 antibody (50266-RP02; Sino Biological, Beijing, China) (1:1000).

Techniques: Knockdown, Western Blot, Injection, Control, Fluorescence, Expressing, Quantitative RT-PCR